Electron Microscopy Sample Preparation
Electron microscopy (EM) is a powerful imaging technique that allows for visualization of structures at the nanometer or even atomic scale. However, proper sample preparation is critical because electron beams interact strongly with matter, and biological or delicate materials can be easily damaged or distorted.
1. Types of Electron Microscopy
Transmission Electron Microscopy (TEM): Electrons pass through thin specimens, providing detailed internal structural information.
Scanning Electron Microscopy (SEM): Electrons scan the surface of specimens, producing 3D-like images of surface morphology.
2. General Considerations
Samples must withstand a high vacuum environment.
Samples need to be electron-dense or treated to enhance electron contrast.
Preparation techniques vary depending on whether the sample is biological, material, or nanostructured.